By Aharon Oren (auth.), Russell H. Vreeland (eds.)
This e-book is designed to be a protracted time period occupation reference. The chapters current sleek methods. this can be a how-to-book with a distinction. those chapters:
- show the historical past information regarding operating with salt loving organisms,
- are loaded with information regarding how experiments are carried out lower than excessive salt,
- supply information regarding analyses that paintings lower than those stipulations and those who would possibly not,
- current a variety of info from laboratory designs to apparatus used or even to easy anecdotal tricks that may merely come from event.
Microbiological education focuses mostly at the progress, the dealing with and the examine of the microbes linked to people and animals. but the most important percentage of the Earth’s microbiota lives in saline environments corresponding to the Oceans, saline deserts and terminal hypersaline environments. This desire for salt should be intimidating for these drawn to coming into the sphere or for these attracted to figuring out how such examine is accomplished.
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Additional info for Advances in Understanding the Biology of Halophilic Microorganisms
ATCC Medium 1279, 1302, and 1453. (Bacillus, Halobacillus, Halomonas, Salibacillus, Salinibacter) has approximately 10 % salinity (Rodriguez-Valera et al. 1980; Quesada et al. 1983; Caton et al. 2004). 1). These media support the growth of a wide range of haloarchaea including Haloarcula, Halobacterium, Halococcus, Haloferax,and Halorubrum. For some media, NaCl is supplemented with other salts in an effort to mimic the composition of concentrated seawater. These media are best suited for organisms from solar salterns fed with seawater or other thalassohaline waters and soils.
Oren recognized in the waters of Mono Lake (Jiang et al. 2004). Metagenomic studies of the crystallizer brine of the Alicante, Spain saltern resulted in the elucidation of the sequence of halophilic phage EHP-1 (Santos et al. 2007). In the past few years a number of in-depth studies of the viral communities in hypersaline brines have been published. 02 μm pore size filters and fluorescent staining with SYBR Gold. Transmission electron microscopy of viruses collected by polyethylene glycol precipitation showed a great morphological diversity of viruses, including spindleshaped, head-and-tail, and several novel viral morphologies (Sime-Ngando et al.
Halomonas elongata was found to grow well with NaCl replaced by NaBr or NaNO3 , but not NaI or Na2 SO4 , while Deleya halophila could use NaBr, Na2 SO4 , and Na2 S2 O3 , but not other sodium salts (Vreeland and Martin 1980; Quesada et al. 1987). Vibrio costicola was able to grow on NaBr, NaMO4 , NaPO4 , and Na2 SO4 , with weak growth on LiCl, KCl, and MgCl2 (Flannery et al. 1952). In a study of 168 halophilic bacterial isolates, 75 strains required NaCl, while 21 strains grew well on media with 1–4 M KCl (Onishi et al.
Advances in Understanding the Biology of Halophilic Microorganisms by Aharon Oren (auth.), Russell H. Vreeland (eds.)