Download PDF by P.G. LeFevre: Active Transport through Animal Cell Membranes

By P.G. LeFevre

ISBN-10: 3211803874

ISBN-13: 9783211803875

ISBN-10: 3709157684

ISBN-13: 9783709157688

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G. LEFEVRE, Active Transport through Animal Cell Membranes actual uptake of fluoride. MALM also noted that the Qll1 of the actual uptake denoted a diffusion process. Nevertheless, RUNNSTROM'S line of argument was resurrected by AUBEL and SJULMAJSTER (1950): they found that E. coli respiration was inhibited by F- in the presence of succinate, and interpreted this to mean that the succinate permitted the F- to enter the cell. In the absence of glucose. fluoride plus either malonate or azide also proved to be effective inhibitory combinations; but neither of the latter two agents assisted F- in inhibition of glucose oxidation.

This phenomenon will be considered later in connection with active water transport and osmoregulation in mammalian tissues. - The study of K42 exchange (MUDGE 1953) gave further insight into the picture. Aerobically, the kinetics indicated at least two intracellular fractions of differing exchangeability, but even the slower compartment had a half-time of only about 25 minutes. However, with 0" deprivation or DNP treatment, a significant fraction of the kidney-slice K became practically non-exchangeable.

Both pyruvate and ATP. 37 "Cyclophorase" (nuclei and mitochondria) Mitochondria Untreated; 0° C . ketogluterate, 5 mM. . and incubated 15 min. at aerobically . anaerobically . . . 1 0° C.. . :Wu C. . Na+ is here removed from medium, while almost none is lost from particles. "Cyclophorase" preparations all incubated 30 minutes at 20°C. 5 mM. ~aHC03 STANBURY and MUDGE (1953) attempted to analyse the relation of the mitochondrial K+ maintenance to the metabolic activity in similar preparations from rabbit liver.

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Active Transport through Animal Cell Membranes by P.G. LeFevre


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